Johnathan+Koper

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DNA Homework Question:
Did everyone in your group have about the same extraction results? Why is this the case? How is the amount of DNA that you extracted affected by your day to day activities?

Everyone in our group did not extract the same amount of DNA, because there wasn't the same amount of DNA in the test tube, also because of the amount of cell lysis solution that was added and the amount of alcohol that was added. It could also be possible that one of the group members made a mistake at one point. The amount of DNA that is extracted would be affected by day to day activities by what you eat or if you have braces where as you would be constantly scraping cells from your cheeks. So basically it all depends on what day to day activities go on that affect the amount of cells that would be able to be extracted.

DNA Replication Summary
DNA replication starts in the origin of replication. An enzyme called Helicase comes in and un-winds the double stranded helix into the leading strand and the lagging strand. Then single stranded binding proteins come in and attach to both strands to ensure that they will not go back together. On the leading strand, an RNA polymerase comes in and adds an RNA primer to give DNA Polymerase III a starting point to start replicating the strand. Since DNA Polymerase III can only add nucleotides from the 3 prime to 5 prime end, the leading strand is replicated faster hence its name. On the lagging strand, the RNA Polymerase adds a series of short segment called Okazaki fragments to the strand. When DNA Polymerase III reaches the next Okazaki fragment, it’s replaced by DNA Polymerase I, which removes the RNA and replaces it with DNA. DNA Ligase then attaches to the strand and fills in the empty spots to form a phosphodiester bond. = DNA Infographic =